Device

Part:BBa_K4375021:Design

Designed by: Zska Csorba   Group: iGEM22_ELTE   (2022-09-29)


Blue light inducible production of mCherry (BLADE)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 29
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1009
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 991


Design Notes

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Source

Oliinyk, O.S., Shemetov, A.A., Pletnev, S. et al. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun 10, 279 (2019). https://doi.org/10.1038/s41467-018-08050-8

References